Rapid extraction of fungal DNA for PCR amplification

Rapid extraction of fungal DNA for PCR amplification.

Polymerase chain reaction (PCR) is a technique widely used in fungal research. One of its advantages is the ability to amplify very small amounts of DNA, in the picograms range, even in the presence of diverse contaminants. In spite of this, most of the extraction protocols of fungal DNA are designed for the obtention of microgram amounts of very pure DNA, requiring the establishment of relativ...

An Efficient Method For DNA Extraction From Paraffin Wax Embedded Tissues For PCR Amplification Of Human And Viral DNA

  Background and Objective: Formalin-fixed paraffin-embedded tissues are a valuable source of DNA for molecular studies. We designed and optimized an efficient procedure for DNA extraction from formalin-fixed paraffin embedded tissues. Materials and Methods: Seventy three blocks of cervical paraffin-embedded tissues were investigated. DNA was extracted using 45 minutes boiling in alkaline sol...

Modified DNA Extraction for Rapid PCR Detection of Methicillin-Resistant Staphylococci

Nosocomial infection caused by methicillin-resistant staphylococci poses a serious problem in many countries. The aim of this study was to rapidly and reliably detect methicillin-resistant-staphylococci in order to suggest appropriate therapy. The presence or absence of the methicillin-resistance gene in 115 clinical isolates of Staphylococcus aureus and 50 isolates of Coagulase Negative Staphy...

Rapid mini-preparation of fungal DNA for PCR.

Rapid DNA Extraction for Screening Soil Filamentous Fungi Using PCR Amplification

A simple and rapid procedure for efficiently isolating fungi DNA suitable for use as a template for PCR amplification and other molecular assays is described. The main advantages of the method are: (1) the mycelium is directly recovered from Petri-dish cultures; (2) the technique is rapid and relatively easy to perform , and (3) it allows for processing of around 50 samples during a single day;...